Synthesis, Characterization and Antimicrobial Screening Mixed-Ligand Cu(II) and Zn(II) Complexes; DNA Binding Studies on Cu(II) Complex

Several mixed ligand Cu(II), Zn(II) complexes using (benzylidenethiourea) (obtained by the condensation of benzaldehyde and thiourea) as the primary ligand and (acetamide or thioacetamide) as an additional ligand were synthesized and characterized analytically and spectroscopically, magnetic susceptibility and molar conductance measurements, as well as by UV-Vis and IR spectroscopy. The interaction of the complexes with calf thymus (CT) DNA was studied using absorption spectra, while the concentration of DNA in gel electrophoresis remained constant at 10 μl. They exhibit absorption hypochromicity increased during the binding of the complexes to calf thymus DNA. The complexes show enhanced antimicrobial activities complexes with the free ligand. A theoretical treatment of the formation of complexes in the gas phase was studied. This was done by using the HYPERCHEM-6 program for the Molecular mechanics and Semi-empirical calculations.